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Levels of ycf1 transcription are highly variable throughout the chloroplast genome in plants. Ycf1 is thought to be necessary for efficient and accurate chromosomal DNA replication. Many algae, Bacteria, and lower plants have chimeric Ycf1 genes consisting of protein-coding sequences from two different origins. In flowering plants, most ycf1 genes encode polypeptides with a single domain, RNAse H-like, which belongs to the group of deoxyribonuclease enzymes that process DNA prereplication intermediates. Deletion of ycf1 has been observed in many angiosperm chloroplast genomes, and so far, this gene has only been found in angiosperm chloroplasts (Fig. 2). Studies have shown that ycf1 knock-out mutant A. thaliana plants are viable but show retarded growth and reduced greening time [103].
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